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HASPIN encodes a serine/threonine kinase that specifically phosphorylates histone H3 at threonine 3 (H3T3ph) during mitosis, an essential step in chromosome alignment and segregation. Their widespread use in signal transduction, drug discovery, and gene function analysis makes them an ideal host for studying mitotic regulators such as HASPIN. These cells exhibit an epithelial-like morphology and are acknowledged as a foundational tool in biomedical research owing to their high transfection efficiency, rapid growth, and well-characterized genetic background. The HEK293 parental cell line originates from human embryonic kidney tissue and has been transformed with adenovirus type 5 (Ad5). This polyclonal pool carries heterogeneous gene disruptions introduced by CRISPR/Cas9-mediated genome editing, providing a robust model for investigating HASPIN-dependent cellular processes without the clonal bias inherent in single-cell-derived knockouts. The HASPIN Knockout HEK293 Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human embryonic kidney cell line HEK293, engineered for loss-of-function studies of the serine/threonine kinase HASPIN.

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